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KMID : 0385019970130010069
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Korean Journal of Laboratory Animal Science
1997 Volume.13 No. 1 p.69 ~ p.68
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Values of Procollagen Type III Peptide and Hydroxyproline in the Rat Liver Cirrhosis Induced by Dimethylnitrosamine and Carbon Tetrachloride
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Abstract
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This study was carried out to investigate whether the levels of serum procollagen type III peptide and hydroxyproline contents in the liver were correlated to fibrosis during development of cirrhosis in rat livers.
Two groups of rats were dosed with dimethylnitrosamine (10μl/kg body weight) intraperitoneally three times a week for 3 weeks and another two groups of rats were given carbon tetrachloride once a week for 10 weeks(initial dose was 0.08mg per
rat).
Serum procollagen type III peptide levels and hydroxyproline contents in the livers of rats given dimethylnitrosamine and carbon tetrachloride were examined and the fibrotic livers were also observed with light microscope.
@ES The results obtained were as follows;
@EN Liver cirrhosis was fully developed within 3 weeks by dimethylnitrosamine while it was possible within 10 weeks by carbon tetrachloride in the rats. Procollagen type III peptide levels in dimethylnitrosamine treated rat sera were 3 times
higher
than
those of control and carbon tetrachloride treated rats. Hydroxyproline contents in dimethylnitrosamine treated rat liver tissues were also 3 times larger than those of control and carbon tetrachloride treated rats. Hydroxyproline contents in
dimethylnitrosamine treated rat liver tissues were also 3 times larger than those of control and carbon tetrachloride treated rats. Procollagen type III peptide was clearly detected immunohistochemically in the livers of rats given
dimethylnitrosamine
or carbon tetrachloride.
These results indicate that dimethylnitrosamine rapidly induces liver cirrhosis and the levels of the serum procollagen type III peptide and hydroxyproline contents in the liver are diagnostic markers in the liver cirrhosis of rats.
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